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anti cd31  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti cd31
    Anti Cd31, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+catenin/Non-phospho+(Active)+beta-Catenin+(Ser33%2F37%2FThr41)+Rabbit+mAb/pmc12972984-356-30-38
    Average 95 stars, based on 138 article reviews
    anti cd31 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Macrophage β-catenin-Ihh axis induces hepatic stellate cell activation and fibrosis in metabolic dysfunction-associated steatohepatitis.
    Article Snippet: .. Immunofluorescence By using rat monoclonal antibody for F4/80 (Cat#: sc-52664, dilution 1:50, Santa Cruz Biotechnology, California) as well as rabbit monoclonal antibody for active β-catenin (nonphosphorylated serine 33/37/threonine41) (Cat#: 8814, 1:200 dilution, Cell Signaling Technology, Massachusetts) or anti-Ihh antibody (Cat#: ab39634, 1:100 dilution, Abcam, Massachusetts), the double-positive macrophages for F4/80 and A-β-catenin or Ihh were successfully identified. .. The α-SMA immunofluorescence staining used rabbit monoclonal antibody α-SMA (Cat#: 19245, 1:200 dilution, Cell Signaling Technology).

    other:

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: SDS PAGE and Western blotting Total protein was extracted from cells and tissues using Tortex lysis buffer (20 mM HEPES, pH 7.9, 350 mM NaCl, 20% v/v glycerol, 1% NP-40, 1 mM MgCl2, 0.5 mM EDTA, 0.1 mM EGTA) with freshly added protease inhibitor (Roche) and phosphatase inhibitor (Roche).

    Incubation:

    Article Title: NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary Adenomas.
    Article Snippet: Membranes were blocked with 5% non- fat milk in TBST (Beyotime, P0231) for 1 h at room temperature, followed 15824934, 2026, 7, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .71097 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [01/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 20Journal of Cellular and Molecular Medicine, 2026 by incubation with primary antibodies overnight at 4°C: GR/ NR3C1 (Cell Signalling Technology, CST, #3660, rabbit monoclonal, 1:1000), phospho- GR- Ser211 (CST, #4161, rabbit monoclonal, 1:1000), β- catenin (CST, #8480, rabbit monoclonal, 1:1000), GSK3β (CST, #9315, rabbit monoclonal, 1:1000), MMP- 9 (CST, #13667, rabbit monoclonal, 1:1000), E- cadherin (CST, #3195, rabbit monoclonal, 1:1000), N- cadherin (CST, #13116, rabbit monoclonal, 1:1000), β- actin (Abcam, ab8226, mouse monoclonal, 1:5000) and Lamin B1 (Abcam, ab133741, rabbit monoclonal, 1:5000). .. The next day, membranes were washed three times with TBST for 10 min each and incubated with HRPconjugated secondary antibodies for 1 h at room temperature (goat anti- rabbit IgG, CST, #7074, 1:5000; goat anti- mouse IgG, CST, #7076, 1:5000).

    Article Title: SNHG5 enhances colorectal cancer metastasis through RNA–protein interaction with GNB2 and activation of canonical Wnt signaling
    Article Snippet: .. Paraffin-embedded liver sections (5 μm) were deparaffinized in xylene, rehydrated through a graded ethanol series, and subjected to endogenous peroxidase blocking with 3 % hydrogen peroxide for 10 min. Antigen retrieval was carried out in citrate buffer (pH 6.0) using microwave heating for 15 min. After cooling to room temperature, sections were blocked with 5 % goat serum for 30 min and incubated overnight at 4 °C with primary antibodies against Ki67 (1:200, Abcam, ab15580), β-catenin (1:100, CST, #8480), E-cadherin (1:100, CST, #3195), and Vimentin (1:200, Abcam, ab92547). .. HRP-conjugated secondary antibodies (1:200, Zsbio, China) were applied for 30 min at room temperature, and signals were developed with DAB.

    Article Title: FGF18 Ameliorates HIV-1 Tat-Induced Blood-Brain Barrier Disruption via Wnt/β-Catenin Signaling.
    Article Snippet: Dysregulated expression of tight junction proteins and compromise of blood‐brain barrier (BBB) integrity are associated with pathological progression of HIV‐1‐associated neurocognitive disorders (HAND).. Fibroblast growth factor‐18 (FGF18), an important member of the fibroblast growth factor (FGF) family, has been considered a neuroprotectant with a wide range of physiological functions.. However, the effects of FGF18 in HAND have not been reported previously.

    Blocking Assay:

    Article Title: SNHG5 enhances colorectal cancer metastasis through RNA–protein interaction with GNB2 and activation of canonical Wnt signaling
    Article Snippet: .. Paraffin-embedded liver sections (5 μm) were deparaffinized in xylene, rehydrated through a graded ethanol series, and subjected to endogenous peroxidase blocking with 3 % hydrogen peroxide for 10 min. Antigen retrieval was carried out in citrate buffer (pH 6.0) using microwave heating for 15 min. After cooling to room temperature, sections were blocked with 5 % goat serum for 30 min and incubated overnight at 4 °C with primary antibodies against Ki67 (1:200, Abcam, ab15580), β-catenin (1:100, CST, #8480), E-cadherin (1:100, CST, #3195), and Vimentin (1:200, Abcam, ab92547). .. HRP-conjugated secondary antibodies (1:200, Zsbio, China) were applied for 30 min at room temperature, and signals were developed with DAB.

    Immunoprecipitation:

    Article Title: Injectable Cu 2+ loaded rhein hydrogel for cross-amplifying osteosarcoma apoptosis and cuproptosis epitranscriptomically.
    Article Snippet: Osteosarcoma (OS) remains a formidable clinical challenge due to its aggressive nature, early metastasis, and resistance to conventional therapies.. Here, we report an injectable Cu2+-loaded rhein hydrogel (RCugel) that integrates sonodynamic therapy (SDT) and cuproptosis with RNA epitranscriptomical regulation for OS treatment.. After administration, RCugel released Cu2+ and rhein to trigger cuproptosis and generated singlet oxygen to induce apoptosis under sonication, respectively.

    Isolation:

    Article Title: Injectable Cu 2+ loaded rhein hydrogel for cross-amplifying osteosarcoma apoptosis and cuproptosis epitranscriptomically.
    Article Snippet: Osteosarcoma (OS) remains a formidable clinical challenge due to its aggressive nature, early metastasis, and resistance to conventional therapies.. Here, we report an injectable Cu2+-loaded rhein hydrogel (RCugel) that integrates sonodynamic therapy (SDT) and cuproptosis with RNA epitranscriptomical regulation for OS treatment.. After administration, RCugel released Cu2+ and rhein to trigger cuproptosis and generated singlet oxygen to induce apoptosis under sonication, respectively.



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